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Association between the angiotensin-converting enzyme (insertion/deletion) and angiotensin II type 1 receptor (A1166C) polymorphisms and breast cancer among Brazilian women

Molecular Gynaecology Laboratory, Department of Gynaecology, Federal University of São Paulo, Rua Pedro de Toledo, 781 — 4th Floor, São Paulo, 04039-032 SP, Brazil, silaac{at}biofis.epm.br

Samuel Marcos Ribeiro de Noronha

Molecular Gynaecology Laboratory, Department of Gynaecology, Federal University of São Paulo, Rua Pedro de Toledo, 781 — 4th Floor, São Paulo, 04039-032 SP, Brazil

Naiara Correa Nogueira-de-Souza

Molecular Gynaecology Laboratory, Department of Gynaecology, Federal University of São Paulo, Rua Pedro de Toledo, 781 — 4th Floor, São Paulo, 04039-032 SP, Brazil

Cristina Valleta de Carvalho

Molecular Gynaecology Laboratory, Department of Gynaecology, Federal University of São Paulo, Rua Pedro de Toledo, 781 — 4th Floor, São Paulo, 04039-032 SP, Brazil

Ana Maria Massad Costa

Molecular Gynaecology Laboratory, Department of Gynaecology, Federal University of São Paulo, Rua Pedro de Toledo, 781 — 4th Floor, São Paulo, 04039-032 SP, Brazil

José Juvenal Linhares

Molecular Gynaecology Laboratory, Department of Gynaecology, Federal University of São Paulo, Rua Pedro de Toledo, 781 — 4th Floor, São Paulo, 04039-032 SP, Brazil

Mariano Tamura Vieira Gomes

Molecular Gynaecology Laboratory, Department of Gynaecology, Federal University of São Paulo, Rua Pedro de Toledo, 781 — 4th Floor, São Paulo, 04039-032 SP, Brazil

Ismael Dale Cotrim Guerreiro da Silva

Molecular Gynaecology Laboratory, Department of Gynaecology, Federal University of São Paulo, Rua Pedro de Toledo, 781 — 4th Floor, São Paulo, 04039-032 SP, Brazil

Introduction.We evaluated the association between components of the renin-angiotensin system and the development of breast cancer in a case-control study by means of angiotensin-converting enzyme (ACE) insertion/deletion (I/D) and angiotensin II type 1 (AT ₁)-receptor A1166C polymorphisms.

Methods. Genotyping was performed by PCR-RFLP (restriction fragment length polymorphism) or PCR (polymerase chain reaction) using genomic DNA extracted from buccal cells of subjects with (101 cases) or without (307 controls) breast cancer.

Results.The frequencies of genotypes for ACE were: DD, ID and II (in %: cases: 60; 20; 20; controls: 46; 37; 17; p=0.019, ²); and for AT₁receptor were:AA,AC and CC (in %: cases: 65; 30; 5; controls: 51; 44; 5; p=0.114, ²).The results suggested that the A1166C polymorphism was not associated with breast cancer risk. On the other hand, for the ACE (I/D), there seemed to be different risks for cancer between cases and controls.

Conclusions.The ID genotype was less frequently associated with the disease than were the DD or II; that is, women with the ID genotype were 3.1 times less likely to develop breast cancer than those with the other genotypes.The ID genotype might be protective against breast cancer and the ACE (I/D) polymorphism a possible target for developing genetic markers for breast cancer.

Key Words: angiotensin-converting enzyme • angiotensin II type 1 receptor • breast cancer • genotyping • polymorphisms

Journal of Renin-Angiotensin-Aldosterone System, Vol. 10, No. 1, 51-58 (2009)
DOI: 10.1177/1470320309102317


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