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Differences Between Cardiac and Arterial Fibrosis and Stiffness in Aldosterone-Salt Rats: Effect of Eplerenone

INSERM U572 Paris, France

Nathalie Mercier

INSERM U684 Vandoeuvre-les-Nancy, France

Carlos Labat

INSERM U684 Vandoeuvre-les-Nancy, France

Athanase Benetos

INSERM U684 Vandoeuvre-les-Nancy, France

Michel E Safar

The Diagnosis Center, Hôtel-Dieu Hospital, Paris, France, michel.safar{at}htd.aphp.fr

Claude Delcayre

INSERM U572 Paris, France

Patrick Lacolley

INSERM U684 Vandoeuvre-les-Nancy, France

Background. Previous experiments have studied separately the development of either cardiac or aortic fibrosis and stiffness in aldosterone (Aldo)-salt hypertensive rats. Our aim was to determine in vivo the effects of Aldo and the Aldo receptor antagonist eplerenone (Epl) on simultaneous changes in cardiac and arterial structure and function and their interactions.

Methods and Results. Aldo was administered in uninephrectomised Sprague-Dawley rats receiving a high-salt diet from 8 to 12 weeks of age. Three groups of Aldo-salt rats were treated with 1 to 100 mg/kg^-1. d^-1 Epl by gavage. Arterial elasticity was measured by elastic modulus (Einc)-wall stress curves using medial cross-sectional area (MCSA). The cardiac and arterial walls were analysed by histomorphometry (elastin and collagen), immunohistochemistry (EIIIA fibronectin, Fn), and Northern blot (collagens I and III). Aldo caused increased systolic blood pressure (SBP), carotid Einc, MCSA, and EIIIA Fn with no change in wall stress or elastin and collagen densities. No difference in collagen mRNA levels was detected between groups. During the same period, cardiac mass and collagen mRNA and protein levels increased markedly in the myocardial tissue. Epl normalised collagen in the myocardium, Eincwall stress curves, MCSA, and EIIIA Fn in Aldo rats. These dose-dependent effects were not accompanied by a consistent reduction in SBP and cardiac mass.

Conclusions. In exogenous hyperaldosteronism in the rat, Aldo causes independently myocardial collagen and arterial Fn accumulation, the latter being responsible for increased intrinsic carotid stiffness. Epl prevents both cardiac and arterial effects but does not reduce consistently SBP.

Key Words: Arteries • Hypertension • Elasticity • Pharmacology

Journal of Renin-Angiotensin-Aldosterone System, Vol. 7, No. 1, 31-39 (2006)
DOI: 10.3317/jraas.2006.004


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