SAGE Journals Online
Advertisement
Sign In to gain access to subscriptions and/or personal tools.

 

Advanced Search

Journal Navigation

Journal Home

Subscriptions

Archive

Contact Us

Table of Contents

Advertisement

Sign In to gain access to subscriptions and/or personal tools.
Journal of Renin-Angiotensin-Aldosterone System
This Article
Right arrow Abstract Freely available
Right arrow Free Full Text (Free PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Petrov, V. V
Right arrow Articles by Lijnen, P. J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petrov, V. V
Right arrow Articles by Lijnen, P. J
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Transforming growth factor-β1 induces angiotensin-converting enzyme synthesis in rat cardiac fibroblasts during their differentiation to myofibroblasts

Victor V Petrov

Hypertension and Cardiovascular Rehabilitation Unit, Department of Molecular and Cardiovascular Research, Faculty of Medicine, University of Leuven (K.U.Leuven), Belgium

Robert H Fagard

Hypertension and Cardiovascular Rehabilitation Unit, Department of Molecular and Cardiovascular Research, Faculty of Medicine, University of Leuven (K.U.Leuven), Belgium

Paul J Lijnen

Hypertension and Cardiovascular Rehabilitation Unit, Department of Molecular and Cardiovascular Research, Faculty of Medicine, University of Leuven (K.U.Leuven), Belgium, paul.lijnen@ med.kuleuven.ac.be

Objectives Appearance of angiotensin-converting enzyme (ACE) in fibrotic tissue can be the result of the action either of one particular growth factor or of cross-talk between multiple factors. Transforming growth factor-β1 (TGF-β 1) is an effective inducor of the differentiation of cultured fibroblasts to myofibroblasts, which are heterogeneous cells with different phenotypes. The present study investigated whether TGF-β1 is able to induce, in vitro, the differentiation of cultured fibroblasts to myofibroblasts with a phenotype containing ACE.

Design Adult rat cardiac ventricular fibroblasts were obtained from hearts perfused with collagenase. Cells from second passage were always used. Rat cardiac ventricular fibroblasts were incubated with TGF-β1 (10 ng/ml) for seven days.

Cell characterisation was performed using light microscopy and indirect immunostaining. Presence of vimentin, desmin, factor VIII, {alpha}-smooth muscle actin, and ACE was checked with both immunostaining and Western blotting. ACE activity was measured fluorometrically with hippuryl-histidyl-leucine as substrate. Synthesis of DNA was measured as 3H-thymidine incorporation.

Results Fibroblasts contained two types of activity of hip-his-leu degradation, namely a lisinopril-dependent activity (ACE activity) and a lisinopril-independent activity (`ACE-like' activity) which is performed by peptidase(s) other than ACE. The ACE activity constituted approximately 30% of the total activity. TGF-β1 induced an increase in both ACE activity and ACE protein (detected by immunoblotting) by 4.5 ± 0.9- and 6.9 ± 2.0-fold, respectively (p<0.05). This induction of ACE was accompanied by a profound modification of the fibroblasts phenotype, which consisted of a change in cell morphology, an enlargement of cell volume and an increase in cell protein content. TGF-β1 profoundly inhibited 3H-thymidine incorporation and the number of cells in growing cultures. The induction of {alpha}-smooth muscle actin by TGF-β 1 (6.8 ± 2.8-fold increase, p<0.05) simultaneously with these modifications in cell morphology and proliferation indicates the appearance of myofibroblasts. These myofibroblasts did not contain desmin.

Conclusion TGF-β 1 is able to induce the appearance of ACE in cultures of adult rat cardiac ventricular fibroblasts. The appearance of the enzyme is accompanied by the differentiation of fibroblasts to myofibroblasts.

Key Words: fibroblasts • myofibroblasts • transforming growth factor-β1 • angiotensin-converting enzyme • {alpha}-smooth muscle actin • desmin • cell proliferation • cell morphology

References

  • Weber KT Extracellular matrix remodeling in heart failure. A role of de novo angiotensin II generation. Circulation 1997; 96:4065-82.[Free Full Text]
  • Sun Y., Zhang J., Zhang JQ, Ramires FJA. Local angiotensin and transforming growth factor-β1 in renal fibrosis of rats. Hypertension 2000; 35:1078-84.[Abstract/Free Full Text]
  • Border WA, Ruoslahti E. Transforming growth factor-β in disease: The dark side of tissue repair. J Clin Invest 1992; 90: 1-7.[Web of Science][Medline] [Order article via Infotrieve]
  • Sun Y., Cleutjens JPM, Diaz-Arias AA, Weber K. Cardiac angiotensin and myocardial fibrosis in the rat. Cardiovasc Res 1994a; 28: 1423-32.[Abstract/Free Full Text]
  • Schroter-Kermani C., Ochsner-Welpelo I., Kittelberger R.An inhibition ELISA for the quantification of collagen type I and type II in cyanogen bromide-digested tissues using fragments-directed antibodies.Immunol Invest 1990; 19: 475-91.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Danilov SM, Chumachenko PV,AndreevaYV, Printseva OY, Lacis RV Angiotensin converting enzyme expression in the inflammation zone of human myocardial infarction and rat model of skin injury. J Hypertens 1993; 11: S232-S233.[Web of Science]
  • Sun Y., Weber K. Angiotensin-converting enzyme and wound healing in diverse tissues of the rat. J Lab Clin Med 1996a; 127: 94-101.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Yamaguchi H., Kim S., Nishikimi T., Takeuchi K., Takeda T. Contribution of cardiac renin-angiotensin system to ventricular remodelling in myocardial-infarcted rats. J Mol Cell Cardiol 1993; 25:1369-80.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Allen RKA, Chai SY, Dunbar MS, Mendelsohn FAO,In vitro autoradiographic localization of angiotensin-converting enzyme in sarcoid lymph nodes. Chest 1986; 90: 315-20.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Katwa LC, Ratajska A., Cleutjens JPM et al. Angiotensin converting enzyme and kininase-II-like activities in cultured valvular interstitial cells of the rat heart. Cardiovasc Res 1995; 29: 57-64.[Abstract/Free Full Text]
  • Sun Y., Weber KT Angiotensin converting enzyme and myofibroblasts during tissue repair in the rat heart. J Mol Cell Cardiol1996b; 28: 851-8.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Vande Berg JS, Rudolph R., Woodward M. Growth dynamics of cultured myofibroblasts from human breast cancer and nonmalignant contracting cultures. Plast Reconstr Surg 1984; 73: 605-18.[Web of Science][Medline] [Order article via Infotrieve]
  • Vande Berg JS, Rudolph R. Cultured myofibroblasts:a useful model to study wound contraction and pathological contracture. Ann Plast Surg 1985; 14: 111-20.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Darby I., Skalli O., Gabbiani G. {alpha}-smooth muscle actin is transiently expressed by myofibroblasts during experimental wound healing. Lab Invest 1990; 63: 21-9.[Web of Science][Medline] [Order article via Infotrieve]
  • Willems Iemg, Havenith MG, De Mey JGR, Daemen Mjap. The {alpha}-smooth muscle actin-positive cells in healing human myocardial scars. Am J Pathol 1994; 145: 868-75.[Abstract]
  • Masur SK, Dewal HS, Dinh TT, Erenburg I., Petridou, S. Myofibroblasts differentiate from fibroblasts when plated at low density. Proc Natl Acad Sci USA 1996; 33: 4219-23.
  • Schmitt-Graff A., Desmoulière A., Gabbiani G. Heterogeneity of myofibroblast phenotypic features:an example of fibroblastic cell plasticity. Virchovs Arch 1994; 425: 3-24.
  • Skalli O., Seemayer T., Lagage R., Montandon D., Pittet B., Gabbiani G. Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins. Lab Invest 1989; 60: 275-85.[Web of Science][Medline] [Order article via Infotrieve]
  • Sappino AP, Schurch W., Gabbiani G. Biology of disease. Differentiation repertoire of fibroblastic cells: expression of cytoskeletal proteins as marker of phenotypic modulation. Lab Invest 1990; 63:144-61.[Web of Science][Medline] [Order article via Infotrieve]
  • Gabbiani G. Evolution and clinical implications of myofibroblasts concept. Cardiovasc Res 1998; 38: 545-8.[Free Full Text]
  • Sun Y., Ratajska G., Zhou G., Weber KT Angiotensin-converting enzyme and myocardial fibrosis in the rat receiving angiotensin II or aldosterone. J Lab Clin Med 1993; 122: 395-403.[Web of Science][Medline] [Order article via Infotrieve]
  • Ratajska A., Campbell SE, Cleutjens JPM, Weber KT Angiotensin II and structural remodeling of coronary vessels in rats. J Lab Clin Med 1994; 124: 408-15.[Web of Science][Medline] [Order article via Infotrieve]
  • Brilla CG, Zhou G., Rupp H., Maisch B., Weber KT Role of angiotensin II and prostaglandin E2 in regulating cardiac fibroblast collagen turnover. Am J Cardiol 1995; 76: 8D-13D.[CrossRef][Medline] [Order article via Infotrieve]
  • Sun Y., Ramires FJA, Weber KT Fibrosis of atria and great vessels in response to angiotensin II or aldosterone infusion. Cardiovasc Res 1997; 35: 138-47.[Abstract/Free Full Text]
  • Ramires FJA, Sun Y., Weber KT Myocardial fibrosis associated with aldosterone or angiotensin II administration: attenuation by calcium channel blockade.JMol Cell Cardiol 1998; 30: 475-83.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Sharma HS, Van Heugton HAA, Goedbloed MA, Verdouw PD, Lamers JMJ Angiotensin II induced expression of transcription factors precedes increase in transforming growth factor-β1 mRNA in neonatal fibroblasts. Biochem Biophys Res Commun 1994; 205: 105-12.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Fisher SA,Absher M. Norepinephrine and Ang II stimulate secretion of TGF-β by neonatal cardiac fibroblasts in vitro.Am J Physiol 1995; 268: C910-C917.[Web of Science][Medline] [Order article via Infotrieve]
  • Lee AA, Dillmann WH, McCulloch AD, Villarreal FJ Angiotensin II stimulates the autocrine production of transforming growth factor-β1 in adult rat cardiac fibroblasts. J Mol Cell Cardiol 1995; 27: 2347-57.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Campbell SE, Katwa LCAngiotensin II stimulated expression of transforming factor-β in cardiac fibroblasts and myofibroblasts. J Mol Cell Cardiol 1997; 29: 1947-58.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Gray MO, Long CS, Kalinyak JE, Li H-T., Karliner JS Angiotensin II stimulates cardiac myocyte hypertrophy via paracrine release of TGF-β1 and endothelin-1 from fibroblasts. Cardiovasc Res 1998; 40: 352-63.[Abstract/Free Full Text]
  • Moriguchi Y., Matsubara H., Mori Y. et al. Angiotensin II-induced trans-activation of epidermal growth factor receptor regulates fibronectin and transforming growth factor-β synthesis via transcriptional and post-transcriptional mechanisms. Circ Res 1999; 84: 1073-84.[Abstract/Free Full Text]
  • Ronnov-Jessen L., Petersen OW Induction of {alpha}-smooth muscle actin by transforming growth factor-β1 in quiescent human breast gland fibroblasts. Implications for myofibro-blasts generation in breast neoplasia. Lab Invest 1933; 68: 696-707.
  • Potts JD, Runjan RB Epithelial-mesenchymal cell transformation in the embryonic heart can be mediated, in part, by transforming growth factor-β. Develop Biol 1988; 134: 392-401.[CrossRef][Web of Science]
  • Eghbali M., Tomek R., Woods C., Bhambi B. Cardiac fibroblasts are predisposed to convert into myocyte phenotype: specific effect of transforming growth factor-β. Proc Natl Acad Sci USA 1991; 88: 795-99.[Abstract/Free Full Text]
  • Desmoulière A., Geinoz A., Gabbiani F., Gabbiani G. Transforming growth factor-β1 induces {alpha}-smooth muscle actin expression in granulation tissue myofibroblasts and in quiescent and growing cultured fibroblasts. J Cell Biol 1993; 122: 103-111.[Abstract/Free Full Text]
  • Desmoulière A., Tuchweber B., Gabbiani G. Role of the myofibroblast differentiation during liver fibrosis. J Hepatol 1995; 22: 61-4.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Grinnell F. Fibroblasts, myofibroblasts, and wound contraction. J Cell Biol 1994; 124:401-4.[Free Full Text]
  • Desmoulière A. Factors influencing myofibroblasts differentiation during wound healing and fibrosis. Cell Biol International 1995; 19: 471-6.[CrossRef]
  • Diamond JR, Van Goor H., Ding G., Engelmyer E. Myofibroblasts in experimental hydronephrosis. Am J Pathol 1995; 146: 121-9.[Abstract]
  • Gressner AM Transdifferentiation of hepatic stellate cells (Ito cells) to myofibroblasts: a key event in hepatic fibrogenesis. Kidney Int 1996; 49: S39-S45.
  • Sigel AV, Centrella M., Eghbali-Webb M. Regulation of proliferative response of cardiac fibroblasts by transforming growth factor-β. J Mol Cell Cardiol 1996; 28: 1921-9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Yokozeki M., Moriyama K., Shimokawa H., Kuroda T. Transforming growth factor-β1 modulates myofibroblastic phenotype of rat palatal fibroblasts in vitro. Exp Cell Res 1997; 231:328-36.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Eghbali M. Cardiac fibroblasts: function, regulation of gene expression, and phenotypic regulation. Basic Res Cardiol 1992; 87: 183-9.[Web of Science][Medline] [Order article via Infotrieve]
  • Brilla CG, Zhou G., Matsubara L.,Weber KT Collagen metabolism in cultured adult rat cardiac fibroblasts: response to angiotensin II and aldosterone. J Mol Cell Cardiol 1994; 26: 809-20.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • De Young MB, Giannattasio B., Scarpa A. Isolation of calcium-tolerant atrial and ventricular myocytes from adult rat heart. In: Biomembranes. Fleischer S, Fleischer B, editors. AP, 1989; 662-76.
  • Lijnen P., Fagard R., Petrov V. Proliferation of human peripheral blood mononuclear cells during calcium channel blockade. Am J Hypertens 1998; 11: 1461-68.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Friedland J., Silverstein E.A sensitive fluorimetric assay for serum angiotensin-converting enzyme. Am J Clin Pathol 1976; 66: 416-24.[Web of Science][Medline] [Order article via Infotrieve]
  • Petrov V., Fagard R., Lijnne P. Effect of protease inhibitors on angiotensin-converting enzyme activity in human T-lymphocytes. Am J Hypertens 2000; 13: 535-539.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Simpson P., McGrath A., Savion S. Myocyte hypertrophy in neonatal rat heart cultures and its regulation by serum and by catecholamines. Circ Res 1982; 51: 787-801.[Abstract/Free Full Text]
  • Moulin V., Castilloux G.,Auger FA et al. Modulated response to cytokines of human wound healing myofibroblasts compared to dermal fibroblasts. Exp Cell Res 1998; 238: 283-93.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Diglio C., Grammas P., Giacomelli F., Wiener J. Rat heart-derived endothelial and smooth muscle cell cultures: isolation, cloning and characterization. Tissue Cell 1988; 20: 477-92.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Butt RP, Laurent GJ, Bishop JE Collagen production and replication by cardiac fibroblasts is enhanced in response to diverse classes of growth factors. Eur J Cell Biol 1995; 68: 330-5.[Web of Science][Medline] [Order article via Infotrieve]
  • Agocha A., Sigel AV, Eghbali-Webb M. Characterization of adult human heart fibroblasts in culture: a comparative study of growth, proliferation and collagen production in human and rabbit cardiac fibroblasts and their response to transforming growth factor-beta1. Cell Tissue Res 1997; 288: 87-93.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Hooper NM Angiotensin converting enzyme: how reliable is the fluorimetric assay with benzoyl-Gly-His-Leu as substrate? Biochem J 1990; 270: 840-1.[Web of Science][Medline] [Order article via Infotrieve]
  • Sun Y., Diaz-Arias AA, Weber KT Angiotensin-converting enzyme, bradykinin, and angiotensin II receptor binding in rat skin, tendon, and heart valves: An in vitro, quantitative autoradiographic study. J Lab Clin Med 1994b; 123: 372-7.[Web of Science][Medline] [Order article via Infotrieve]
  • Vaghy PL, Russell JS, Lantry LE, Stephens RE, Ward PE Angiotensin and bradykinin metabolism by peptidases identified in cultured human skeletal muscle myocytes and fibroblasts. Peptides 1995; 16:1367-73.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Dostal DE, Rothblum KN, Conrad KM, Cooper GR, Baker KM Detection of angiotensin I and II in cultured rat cardiac myocytes and fibroblasts.Am JPhysiol 1992; 263: C851-C863.[Web of Science][Medline] [Order article via Infotrieve]
  • Van Kesteren CAM, Saris JJ, Dekkers DHW et al. Cultured neonatal rat cardiac myocytes and fibroblasts do not synthesize renin or angiotensinogen: evidence for stretch-induced cardiomyocyte hypertrophy independent of angiotensin II. Cardiovasc Res 1999; 43:148-56.[Abstract/Free Full Text]
  • Booz GW, Dostal DE, Singer HA, Baker KM Involvement of protein kinase C and Ca2+ in angiotensin II-induced mitogenesis of cardiac fibroblasts. Am J Physiol 1994; 267: C1308-C1318.[Web of Science][Medline] [Order article via Infotrieve]
  • Schorb W., Conrad KM, Singer HA, Dostal DE, Baker KM Angiotensin II is a potent stimulator of MAP-kinase activity in neonatal rat cardiac fibroblasts. J Mol Cell Cardiol 1995; 27: 1151-60.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Grohe C., Kahlert S., Lobbert K. et al. Angiotensin converting enzyme inhibition modulates cardiac fibroblasts growth. J Hypertens 1998; 16: 377-84.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Lijnen P., Petrov VV, Fagard R. Stimulation of collagen secretion by angiotensin II and transforming factor-β in rat cardiac fibroblasts. Eur Heart J 1998; 19: 488.
  • Petrov V., Fagard R., Lijnen P. Effect of angiotensin II and transforming growth factor-β on collagen secretion in cardiac fibroblasts. Am J Hypertens 1998; 11: 146A.[CrossRef]
  • Sun Y., Weber KT Cell expressing angiotensin II receptors in fibrous tissue of rat heart. Cardiovasc Res 1996c; 31: 518-25.[Abstract/Free Full Text]
  • Katwa LC, Campbell SE,Tyagy SC, Lee SJ, Cicila GT, Weber KT Cultured myofibroblasts generate angiotensin peptides de novo. J Mol Cell Cardiol 1997; 29: 1375-86.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Miller JJ, Changaris DG, Levy RS Conversion of angiotensin I to angiotensin II by cathepsin A isoenzymes of porcine kidney. Biochem Biophys Res Commun 1988; 154: 1122-9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Sasaguri M., Maeda H., Noda K. et al. Purification and characterization of a kinin- and angiotensin II-forming enzyme in the dog heart. J Hypertens 1997; 15: 675-82.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Grise C., Boucher R., Thibault G., Genest J. Formation of angiotensin II by tonin from partially purified human angiotensinogen. Can J Biochem 1981; 59: 250-5.[Web of Science][Medline] [Order article via Infotrieve]
  • Wintroub BU, Schechter NB, Lazarus GS, Kaempfer CE, Schwartz LB Angiotensin I conversion by human and rat chymotryptic proteinases. J Invest Dermatol 1984; 83: 336-9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Urata H., Boehm KD, Philip A. et al. Cellular localization and regional distribution of an angiotensin II-forming chymase in the heart. J Clin Invest 1993; 91: 1269-81.[Web of Science][Medline] [Order article via Infotrieve]
  • Urata H., Nishimura H., Ganten D. Chymase-dependent angiotensin II-forming system in humans. Am J Hypertens 1996; 9: 227-84.
  • Reilly CF,Tewksbury DA, Schechter NM,Travis J. Rapid conversion of angiotensin I to angiotensin II by neutrophil and mast cell proteinases. J Biol Chem 1982; 257: 8619-22.[Abstract/Free Full Text]
  • Botsein GR, Sherer GK, Leroy C. Fibroblast selection in scleroderma.An alternative model of fibrosis. Arthritis Rheum 1982; 25: 189-95.[Web of Science][Medline] [Order article via Infotrieve]
  • Breen E., Falco VM, Absher M., Cutroneo KR Subpopulations of rat lung fibroblasts with different amounts of type I and type III collagen mRNAs. J Biol Chem 1990; 265: 6286-90.[Abstract/Free Full Text]
  • McCulloch CAG, Bordin S. Role of fibroblast subpopulations in periodontal physiology and pathology. J Periodont Res 1991; 26: 144-54.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Derdak S., Penney DP, Keng P., Felch ME, Brown D., Phipps RP Differential collagen and fibronectin production by Thy1+ and Thy1- lung fibroblasts subpopulations. Am J Physiol 1992; 263: L283-L290.[Web of Science][Medline] [Order article via Infotrieve]
  • Desmoulière A., Rubbia-Brandt L.,Abdiu A., Walz T., Magieira-Coelho A., Gabbiani G. {alpha}-Smooth muscle actin is expressed in a subpopulation of cultured and cloned fibroblasts and is modulated by gamma-interferon. Exp Cell Res 1992; 201: 64-73.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Penney DP, Keng PC, Derdak S., Phipps RP Morphologic and functional characteristics of subpopulations of murine lung fibroblasts grown in vitro.Anatomical Record 1992; 232: 432-43.[CrossRef][Medline] [Order article via Infotrieve]
  • Fries KM, Blieden T., Looney RJ et al. Evidence of fibroblasts heterogeneity and the role of fibroblast subpopulations in fibrosis. Clin Immunol Immunopathol 1994a; 72: 283-92.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Fries KM, Felch ME, Phipps RP Interleukin-6 an autocrine growth factor for murine lung fibroblast subsets. Am J Respir Cell Mol Biol 1994b; 11: 552-60.[Abstract]
  • Silvera MR, Sempowski GD, Phipps RP Expression of TGFβ isoforms by Thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGF-β as a regulator of IL-1-dependent stimulation if IL-6. Lymphokine and Cytokine Res 1994; 13: 277-85.
  • Klett CPR, Palmer AA, Dirig DM, Gallagher A-M., Riosecco-Camacho N., Printz MP Evidence for differences in cultured left ventricular fibroblast populations isolated from spontaneously hypertensive and Wistar-Kyoto rats. J Hypertens 1995a; 13: 1421-31.[Web of Science][Medline] [Order article via Infotrieve]
  • Klett CPR, Palmer A., Gallagher A-M., Riosecco-Camacho N., Printz MP Differences in cultured cardiac fibroblast populations isolated from SHR and WKY rats. Clin Exp Pharmacol Physiol 1995b; S265-S267.
  • Sempowski GD, Derdak S., Phipps RP Interleukin-4 and Interpheron-gamma discordantly regulate collagen biosynthesis by functionally distinct lung fibroblasts subsets. J Cell Physiol 1996; 167: 290-6.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Strittmatter SM, Snyder SH Characterization of angiotensin converting enzyme by 3H-captopril binding. Mol Pharmacol 1986; 29: 142-8.[Abstract]
  • Alexandrow MG, Moses HL Transforming growth factor-β and cell cycle regulation. Cancer Res 1995; 55: 1452-7.[Free Full Text]
  • Gibbons GH, Pratt RE, Dzau VJ Vascular smooth muscle cell hypertrophy vs. hyperplasia. Autocrine transforming growth factor-β1 expression determines growth response to angiotensin II. J Clin Invest 1992; 90: 456-61.[Web of Science][Medline] [Order article via Infotrieve]
  • Lijnen P., Petrov V. Antagonism of the renin-angiotensin-aldosterone system and collagen metabolism in cardiac fibroblasts. Methods Find Exp Clin Pharmacol 1999; 21: 215-25.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Van Krimpen C., Smits JFM, Cleutjens JPM, Debets JJ, Schoemaker RG, Struyker-Boudir HA, Bosman FT, Daemen MJ DNA synthesis in the non-infarcted cardiac interstitium after left coronary artery ligation in the rat heart: effects of captopril. J Mol Cell Cardiol 1991; 23: 1245-53.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Nguyen L., Ward WF, Ts'Ao CH, MolteniA. Captopril inhibits proliferation of human lung fibroblasts in culture: a potential antifibrotic mechanism. Proc Soc Exp Biol Med 1994; 205: 80-8.[CrossRef][Medline] [Order article via Infotrieve]

Journal of Renin-Angiotensin-Aldosterone System, Vol. 1, No. 4, 342-352 (2000)
DOI: 10.3317/jraas.2000.064
Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 Journal of Renin-Angiotensin-Aldosterone SystemHome page
M. Copaja Soto, R. Valenzuela, A. Saldana, M. Paz Ocaranza, J. E. Jalil, C. Vio, P. Lijnen, G. E. Ordenes, R. Vivar Sanchez, S. Lavandero, et al.
Early expression of monocyte chemoattractant protein-1 correlates with the onset of isoproterenol-induced cardiac fibrosis in rats with distinct angiotensin-converting enzyme polymorphism
Journal of Renin-Angiotensin-Aldosterone System, September 1, 2008; 9(3): 154 - 162.
[Abstract] [PDF]

Home page
 J Am Coll CardiolHome page
B. Burstein and S. Nattel
Atrial Fibrosis: Mechanisms and Clinical Relevance in Atrial Fibrillation
J. Am. Coll. Cardiol., February 26, 2008; 51(8): 802 - 809.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
V. Drobic, R. H. Cunnington, K. M. Bedosky, J. E. Raizman, V. V. Elimban, S. G. Rattan, and I. M. C. Dixon
Differential and combined effects of cardiotrophin-1 and TGF-beta1 on cardiac myofibroblast proliferation and contraction
Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1053 - H1064.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
B. Wang, A. Omar, T. Angelovska, V. Drobic, S. G. Rattan, S. C. Jones, and I. M. C. Dixon
Regulation of collagen synthesis by inhibitory Smad7 in cardiac myofibroblasts
Am J Physiol Heart Circ Physiol, August 1, 2007; 293(2): H1282 - H1290.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
I. M.C. Dixon
Much ado about bone marrow stem cells: Role in post-myocardial infarct repair
Cardiovasc Res, September 1, 2006; 71(4): 609 - 611.
[Full Text] [PDF]

Home page
 HypertensionHome page
I. M.C. Dixon and V. Drobic
Gender Dependency in the Pathogenesis of Cardiac Hypertrophy: Effect of Norepinephrine on Transforming Growth Factor-{beta} Release in Female Heart
Hypertension, October 1, 2004; 44(4): 392 - 393.
[Full Text] [PDF]

This Article
Right arrow Abstract Freely available
Right arrow Free Full Text (Free PDF) Free
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Saved Citations
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Request Reprints
Right arrow Add to My Marked Citations
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Right arrow Citing Articles via Scopus
Google Scholar
Right arrow Articles by Petrov, V. V
Right arrow Articles by Lijnen, P. J
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Petrov, V. V
Right arrow Articles by Lijnen, P. J
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Advertisement