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Candesartan decreases the sympatho-adrenal and hormonal response to isolation stress

Section on Pharmacology, NIMH, NIH, Bethesda, MD 20892, USA, ArmandoI{at}intra.nimh.nih.gov, Centro de Investigaciones Endocrinologicas, CONICET, Buenos Aires, Argentina

Andrea Carranza

Centro de Investigaciones Endocrinologicas, CONICET, Buenos Aires, Argentina

Yasuaki Nishimura

Section on Pharmacology, NIMH, NIH, Bethesda, MD 20892, USA

Marta Barontini

Centro de Investigaciones Endocrinologicas, CONICET, Buenos Aires, Argentina

Takeshi Ito

Section on Pharmacology, NIMH, NIH, Bethesda, MD 20892, USA

Juan M Saavedra

Section on Pharmacology, NIMH, NIH, Bethesda, MD 20892, USA

A change from group housing to isolation in unfamiliar metabolic cages represents, for rodents, a significant emotional stress. We studied the effect of candesartan, a peripheral and central angiotensin II AT₁-receptor antagonist, on the hormonal and sympathetic response to acute isolation. We pretreated rats with 1 mg/kg/day candesartan for 13 days via subcutaneously implanted osmotic minipumps, followed by 24-hour isolation in individual metabolic cages. We measured brain, pituitary and adrenal angiotensin II (Ang II) receptor binding by quantitative autoradiography and adrenal hormones and catecholamines by RIA and HPLC. Isolation increased adrenal catecholamines, aldosterone and corticosterone, AT₁-receptor binding in the zona glomerulosa and AT₂-receptor binding in the adrenal medulla. Candesartan pretreatment decreased adrenal catecholamines, aldosterone and corticosterone, AT₁-receptor binding in adrenal zona glomerulosa and medulla, pituitary gland and the hypothalamic paraventricular nucleus, and AT₂-receptor binding in adrenal medulla, but increased AT₂-receptor binding in zona glomerulosa. We conclude that peripheral and central AT₁-receptor blockade with candesartan decreases the sympatho-adrenal and hormonal response to acute stress. Our results indicate that Ang II is an important stress hormone and suggest that blockade of the physiologically active AT ₁-receptors could influence stress-related disorders.

Key Words: renin-angiotensin system • angiotensin receptors • adrenal gland • catecholamines • pituitary gland • corticoids • brain

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Journal of Renin-Angiotensin-Aldosterone System, Vol. 2, No. 1 suppl, S130-S135 (2001)
DOI: 10.1177/14703203010020012301


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Armando, I. Articles by Saavedra, J. M Search for Related Content PubMed Articles by Armando, I. Articles by Saavedra, J. M Social Bookmarking                         What's this?