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Journal of Renin-Angiotensin-Aldosterone System
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Contractile effects of angiotensin peptides in rat aorta are differentially dependent on tyrosine kinase activity

Gheorghe Petrescu

Department of Physiology, petrescugh{at}yahoo.com

Marcel Costuleanu

Department of Pathophysiology

Simona Mihaela Slatineanu

Department of Physiology

Natalia Costuleanu

Department of Biochemistry, University of Medicine and Pharmacy "Gr. T. Popa" Iasi, Romania

Liliana Foia

Department of Biochemistry, University of Medicine and Pharmacy "Gr. T. Popa" Iasi, Romania

Angela Costuleanu

Department of Physiology

It has been suggested that tyrosine kinase activity participates in the regulation of signal transduction associated with angiotensin II (Ang II)-induced pharmaco-mechanical coupling in rat aortic smooth muscle. We further tested the effects of genistein, a tyrosine-kinase inhibitor, and its inactive analogue, daidzein, on angiotensin I (Ang I), angiotensin III (Ang III) and angiotensin IV (Ang IV) contractions, as compared with those on Ang II. Genistein partially inhibited Ang II- and Ang I-induced contractions. The genistein-induced inhibition was more evident on Ang III and especially important on Ang IV contractile effects. Thus, Ang IV- and Ang III-induced contractions seem to be more dependent on tyrosine kinase activity than those evoked by Ang II or Ang I. Daidzein did not significantly affect the contractile effects of any of angiotensin peptides tested. These results clearly suggest that the inhibition of the action of angiotensin peptides actions by genistein is mediated by inhibition of endogenous tyrosine kinase activity. Furthermore, our data show that the type and/or intensity of tyrosine kinase activity is differentially associated with the contractile effects of different angiotensin peptides in rat aorta. Nifedipine, a blocker of membrane L-type Ca2+ channels, strongly inhibited Ang IV-induced contractions. At the same time, it significantly inhibited Ang III contractile effects as compared with Ang II and Ang I contractions. Meanwhile, we observed a close relationship between calcium influx and tyrosine kinase phosphorylation activity under the stimulatory effects of angiotensin peptides. Furthermore, genistein did not significantly influence the phasic contractions induced by angiotensin peptides in Ca2+-free Krebs-Henseleit solution. Thus, it appears that Ca2+ influx, rather than the release of Ca2+ from IP3-sensitive stores, may play a major role in the contractile effects of angiotensin peptides in rat aorta via tyrosine kinase activation. One argument against a direct action of genistein on the Ca 2+ channel itself is that it did not markedly affect the K+-induced contraction (depolarisation) in rat aorta. At the same time, a potential role for tyrosine kinase activity in the process of calcium entry is suggested. An elevation of intracellular calcium via tyrosine kinase-mediated processes may mediate the actions of G-protein coupled receptor agonists in smooth muscle, including angiotensin peptides.

Key Words: angiotensin I • angiotensin II • angiotensin III • angiotensin IV • tyrosine kinase activity • aorta • rat

References

  • Touyz RM, Schiffrin EL Signal transduction mechanisms mediating the physiological and pathophysiological actions of angiotensin II in vascular smooth muscle cells. Pharmacol Rev 2000;52:639-72.[Abstract/Free Full Text]
  • Prescott G., Silversidis DW, Chiu SML, Reudelhuber TL Contribution of circulating renin to local synthesis of angiotensin peptides in the heart. Physiol Genomics 2000;4:67-73.[Abstract/Free Full Text]
  • Saris JJ, van Dijk MA, Kroon I., Schalekamp Madh, Danser AHJ. Functional importance of angiotensin-converting enzyme-dependent in situ angiotensin II generation in the human forearm. Hypertension 2000;35:764-8.[Abstract/Free Full Text]
  • Andre P., Schott C., Nehlig H., Stoclet JC Aortic smooth muscle cells are able to convert angiotensin I to angiotensin II. Biochim Biophys Res Commun 1990;173(3):1137-42.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Robertson MJ, Cunoosamy MP, Clark KLEffects of peptidase inhibition on angiotensin receptor agonist and antagonist potency in rabbit isolated thoracic aorta. Br J Pharmacol 1992;106(1):166-72.[Web of Science][Medline] [Order article via Infotrieve]
  • Cheng DY, Dewitt BJ, Dent EL, Nossaman BD, Kadowitz PJ Analysis of response to angiotensin IV in the pulmonary vascular bed of the cat. Eur J Pharmacol 1994;261(1-2):223-7.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Li Q., Zhang J., Pfaffendorf M., van Zwieten PA Comparative effects of angiotensin II and its degradation products angiotensin III and angiotensin IV in rat aorta. Br J Pharmacol 1995;116(7):2963-70.[Web of Science][Medline] [Order article via Infotrieve]
  • Nossaman BD, Feng CJ, Kaye AD, Kadowitz PJ Analysis of responses to ANG IV: effect of PD-123319 and DuP-753 in the pulmonary circulation of the rat. Am J Physiol (Lung Cell Mol Physiol 12) 1995;268(2Pt1):L302-L308.[Abstract/Free Full Text]
  • Ferrario CM, Iyer SN Angiotensin-(1-7): a bioactive fragment of the renin-angiotensin system. Regul Pept 1998;78(1-3):13-18.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Catt KJ Angiotensin II receptors. In The renin-angiotensin system. ed. Robertson JS & Nicholls MG pp. 12.1-12.4. LondonNew York: Gower Medical Publishing, 1993.
  • Chaki S., Inagami T. Identification and characterization of a new binding site for angiotensin II in mouse neuroblastoma neuro-2A cells. Biochem Biophys Res Commun 1992;182(1): 388-94.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Näveri L. The role of receptor subtypes in cerebrovascular regulation in the rat. Acta Physiol Scand 1995;155(suppl 630):1-48.[Web of Science][Medline] [Order article via Infotrieve]
  • Nickenig G., Geisen G., Vetter H., Sachiniadis A. Characterization of angiotensin receptors on human skin fibroblasts.JMolMed 1997;75(3):217-22.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Chen L., McNeill JR, Wilson TW, Gopalakrishnan V. Heterogeneity in vascular smooth muscle responsiveness to angiotensin II. Role of endothelin. Hypertension 1995;26(1):83-8.[Abstract/Free Full Text]
  • Alexander RW, Brock TA, Gimbrone MA, Rittenhouse SL Angiotensin II increases inositol triphosphate and calcium in vascular smooth muscle cells.Hypertension 1985;7(3Pt1):447-51.[Abstract/Free Full Text]
  • Berridge MJ Inositol trisphosphate and calcium signalling. Nature 1993;361(6410):315-25.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Marrero MB, Schieffer B., Bernstein KE, Ling BN.Angiotensin II-induced tyrosine phosphorylations in mesangial and vascular smooth muscle cells. Clin Experim Pharmacol Physiol 1996;23(1):83-8.
  • Di Salvo J., Steusloff A., Semenchuk L., Satoh S., Kolquist K., Pfitzer G.Tyrosine kinase inhibitors suppress agonist-induced contraction in smooth muscle. Biochem Biophys Res Commun 1993;190(3):968-74.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Laniyonu AA, Saifeddine M., Yang S.-G, Hollenberg MD Tyrosine kinase inhibitors and the contractile action of G-protein-linked vascular agonists. Can J Physiol Pharmacol 1994;72(9):1075-85.[Web of Science][Medline] [Order article via Infotrieve]
  • Huckle WR, Dy RC, Earp HS Calcium-dependent increase in tyrosine-kinase activity stimulated by angiotensin II. Proc Natl Acad Sci USA 1992;89(18):8837-41.[Abstract/Free Full Text]
  • Timmermans Pbmwm,Wong PC, Chiu AT et al. Angiotensin II receptors and angiotensin II receptor antagonists. Pharmacol Rev 1993; 45(2):205-51.[Web of Science][Medline] [Order article via Infotrieve]
  • Stroth U., Unger T. The renin-angiotensin system and its receptors. J Cardiovasc Pharmacol 1999;33(suppl 1):S21-S28.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Moore AF, Hall HM, Khairallah PA A comparison of the effects of angiotensin II and heptapeptide on smooth muscle (vascular and uterine). Eur J Pharmacol 1976;39(1):101-07.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Catalioto RM, Porchia R., Renzetti AR, Criscuoli M., Subissi A., Giachetti A.Angiotensin II-induced responses in vascular smooth muscle cells: inhibition by non-peptide receptor antagonists. Eur J Pharmacol 1995;280(3):285-92.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Wright JW, Harding JW Brain angiotensin receptor subtypes AT1,AT2, and AT4 and their functions. Regul Pept 1995;59(3):269-95.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Sim MK Degradation of angiotensin I in the endothelium and smooth muscle of the rat aorta. Biochem Pharmacol 1993;45(7):1524-7.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Kramar EA, Harding JW, Wright JW. Angiotensin II- and IV-induced changes in cerebral blood flow. Roles of AT1,AT2, and AT4 receptor subtypes. Regul Pept 1997;68(2):131-8.
  • Brailoiu E.,Todiras M., Margineanu A. et al.TLC characterization of liposomes containing angiotensinogen, angiotensine I, angiotensine II and saralazin. Biomed Chromatogr 1997;11(3):160-3.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Griendling KK, Ushio-Fukai M., Lassègue B., Alexander RW Angiotensin II signaling in vascular smooth muscle. New concepts.Hypertension 1997;29(1Pt2): 366-73.
  • Tsunoda Y. Receptor-operated calcium influx mediated by protein tyrosine kinase pathways. J Recept Sign Transduct Res 1998;18(4-6):281-310.[CrossRef]
  • Johns DW, Sperelakis N. Angiotensin stimulates Ca2+-dependent action potentials in cultured smooth muscle cells. Eur J Pharmacol 1990;187(2):183-91.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Gunther S., Gimbrone RA, Alexander RW Identification and characterization of the high affinity vascular angiotensin II receptor in rat mesenteric artery. Circ Res 1980; 47(2):278-86.[Free Full Text]
  • Moore GJ Designing peptide mimetics. Trends Pharmacol Sci 1994;15(4):124-9.[CrossRef][Medline] [Order article via Infotrieve]
  • Chang RSL, Lotti VJ Angiotensin receptor subtypes in rat, rabbit and monkey tissues: relative distribution and species dependency. Life Sci 1991;49(20):1485-90.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Patel JM, Martens JR, Li YD, Gelband CH, Raizada MK, Block ER.Angiotensin IV receptor-mediated activation of lung endothelial NOS is associated with vasorelaxation. Am J Physiol (Lung Cell Mol Physiol 19) 1998;275(6Pt1):L1061-L1068.[Abstract/Free Full Text]
  • Swanson GN, Hanesworth JM, Sardinia MF et al. Discovery of a distinct binding site for angiotensin II (3-8), a putative angiotensin IV receptor. Regul Pept 1992;40(3):409-19.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Hall KL, Hanesworth JM, Ball AE, Felgenhauer GP, Hosick HL, Harding JW. Identification and characterization of a novel angiotensin binding site in cultured vascular smooth muscle cells that is specific for the hexapeptide (3-8) fragments of angiotensin II, angiotensin IV. Regul Pept 1993;44(2):225-32.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Nishizuka Y. The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature 1984; 308(5961):693-8.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Meloche S., Landry J., Huot J., Houle F., Marceau F., Giasson E. p38 MAP kinase pathway regulates angiotensin II-induced contraction of rat vascular smooth muscle. Am J Physiol 2000;279:741-51.
  • Eguchi S., Iwasaki H., Inagami T. et al. Involvement of PYK2 in angiotensin II signaling of vascular smooth muscle cells. Hypertension 1999;33(1Pt2):201-6.[Abstract/Free Full Text]
  • Seki T.,Yokoshiki H., Sunagawa M., Nakamura M., Sperelakis N. Angiotensin II stimulation of Ca2+-channel current in vascular smooth muscle cells is inhibited by lavendustin-A and LY-294002. Pflugers Arch 1999;437(3):317-23.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Ushio-Fukai M.,Alexander RW,Akers M., Lyons PR, Lassègue B., Griendling KKAngiotensin II receptor coupling to phospholipase D is mediated by the β{gamma} subunits of heterotrimeric G proteins in vascular smooth muscle cells. Mol Pharmacol 1999; 55(1):142-9.[Abstract/Free Full Text]
  • Di Salvo J., Gifford D., Kokkinakis A.ATP- and polyphosphate-mediated stimulation of pp60c-src kinase activity in extracts from vascular smooth muscle. J Biol Chem 1989;264(10):10773-8.[Abstract/Free Full Text]
  • Molloy CJ,Taylor DS, Weber H.Angiotensin II stimulation of rapid protein tyrosine phosphorylation and protein kinase activation in rat aortic smooth muscle cells. J Biol Chem 1993;268 (10):7338-45.[Abstract/Free Full Text]
  • Berk BC Angiotensin II signal transduction in vascular smooth muscle: pathways activated by specific tyrosine kinases.J Am Nephrol 1999;10(suppl 11):S62-68.
  • Yang S.-G, Saifeddine M., LaniyonuA, Hollenberg MD Distinct signal transduction pathways for angiotensin II in guinea pig gastric smooth muscle: differential blockade by indomethacin and tyrosine kinase inhibitors. J Pharmacol Exp Ther 1993;264(2): 958-66.[Abstract/Free Full Text]
  • Akiyama T., Ishida J., Nakagawa S. et al. Genistein, a specific inhibitor of tyrosine-specific protein kinases. J Biol Chem 1987;262(12):5592-5.[Abstract/Free Full Text]
  • Hollenberg MD Tyrosine kinase-mediated signal transduction pathways and the actions of polypeptide growth factors and G-protein-coupled agonists in smooth muscle.Mol Cell Biochem 1995;149/150:77-85.
  • Wijetunge S., Aalkjaer C., Schachter M., Hughes AD Tyrosine kinase inhibitors block calcium channel currents in vascular smooth muscle cells. Biochem Biophys Res Commun 1992;189(3):1620-3.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Thomas WG Regulation of angiotensin II type I (AT1) receptor function. Regul Pept 1999;79(1):9-23.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Leduc I., Haddad P., Giasson E., Meloche S. Involvement of a tyrosine kinase pathway in the growth-promoting effects of angiotensin II on aortic smooth muscle cells. Mol Pharmacol 1995;48(4):582-92.[Abstract]
  • Smith JB Angiotensin-receptor signaling in cultured vascular smooth muscle cells. Am J Physiol 1986;250(5Pt2):F759-F769.[Web of Science][Medline] [Order article via Infotrieve]
  • van Heiningen PN, Batink HD, van Zwieten PAAngiotensin II-induced increase in slowly exchanging 45Ca2+ in relation to contractile responses of rat and guinea-pig aorta. Naunyn Schmiedebergs Arch Pharmacol 1991;344(1):107-13.[Web of Science][Medline] [Order article via Infotrieve]
  • Kusaka M., Sperelakis N. Inhibition of L-type calcium current by genistein, a tyrosine kinase inhibitor, in pregnant rat myometrial cells.BiochimBiophysActa 1995;1240(2):196-200.[Medline] [Order article via Infotrieve]
  • Lee K.-M,Toscas K.,Villereal ML Inhibition of bradykinin- and thapsigargin-induced Ca2+ entry by tyrosine kinase inhibitors. J Biol Chem 1993;268(14):9945-8.[Abstract/Free Full Text]
  • Sargeant P., Farndale RW, Sage O. The tyrosine kinase inhibitors methyl-2,5-dihydroxycinnamate and genistein reduce thrombin-evoked tyrosine phosphorylation and Ca2+ entry in human platelets. FEBS Lett 1993;315(13):242-6.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]

Journal of Renin-Angiotensin-Aldosterone System, Vol. 2, No. 3, 180-187 (2001)
DOI: 10.3317/jraas.2001.025
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