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Journal of Renin-Angiotensin-Aldosterone System
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Macroarray analysis in the hypertrophic left ventricle of renin-dependent hypertensive rats: identification of target genes for renin

Mazen Kurdi

EA 3740, Laboratoire de Pharmacologie, Génomique fonctionnelle dans l'athéro-thrombose, Université Claude Bernard-Lyon1, UFR de Médecine RTH Laennec, rue Guillaume Paradin, 69372 Lyon cedex 08, France

Catherine Cerutti

EA 3740, Laboratoire de Pharmacologie, Génomique fonctionnelle dans l'athéro-thrombose, Université Claude Bernard-Lyon1, UFR de Médecine RTH Laennec, rue Guillaume Paradin, 69372 Lyon cedex 08, France

Jacques Randon

EA 3740, Laboratoire de Pharmacologie, Génomique fonctionnelle dans l'athéro-thrombose, Université Claude Bernard-Lyon1, UFR de Médecine RTH Laennec, rue Guillaume Paradin, 69372 Lyon cedex 08, France

Lilian McGregor

EA 3740, Laboratoire de Pharmacologie, Génomique fonctionnelle dans l'athéro-thrombose, Université Claude Bernard-Lyon1, UFR de Médecine RTH Laennec, rue Guillaume Paradin, 69372 Lyon cedex 08, France

Giampiero Bricca

EA 3740, Laboratoire de Pharmacologie, Génomique fonctionnelle dans l'athéro-thrombose, Université Claude Bernard-Lyon1, UFR de Médecine RTH Laennec, rue Guillaume Paradin, 69372 Lyon cedex 08, France, bricca@lyon. inserm.fr

Introduction The aim of this work was to identify new renin target genes in left ventricular hypertrophy during hypertension.

Materials and methods We compared left ventricle gene expression from four transgenic TGR(mRen2)27 (TG+/-) rats and four non-transgenic littermates (TG-/-) using cDNA macroarray. Hybridisation signals were quantified with a phosphorimager, and normalised to an external scale. Data analysis was performed with Statistical Analysis for Microarrays (SAM 1.21) software. The mRNA levels of candidate genes were determined by semi-quantitative RT-PCR in three different hypertensive rats: TG+/-, spontaneously hypertensive (SHR) and genetically Lyon hypertensive (LH) rats, compared to their respective controls (TG-/-, Wistar-Kyoto, Lyon low blood pressure rats).

Results Out of 1,200 genes present on the macroarray, 233 were reliably measured and only three were overexpressed (Biglycan, β1-adenosine monophosphate-activated protein kinase [AMPK] and amyloid precursor like protein 2 [APLP2]) and 19 were underexpressed in the left ventricle of TG+/compared with TG-/-. APLP2 is a member of the amyloid precursor protein (APP) family. APLP2 and APP mRNA levels were increased in TGR(mRen2)27 but significantly decreased in LH rats, while only APP was increased in SHR rats.

Conclusions We report new genes associated with renin-dependent left ventricular hypertrophy. Moreover, this work shows for the first time that the APP family gene expression could be altered in response to high renin activity and this effect is independent of cardiac remodelling and hypertension.

Key Words: hypertension • cDNA array • gene expression • YWK-II • APLP2 • APP • genetically hypertensive rats • biglycan • AMPK

References

  • Hamet P. Environmentally-regulated genes of hypertension. Clin Exp Hypertens 1996;18:267-78.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Gaballa MA, Goldman S. Ventricular remodeling in heart failure. J Card Fail 2002;8:476-85.[CrossRef]
  • Ruwhof C., van der Laarse A. Mechanical stress-induced cardiac hypertrophy: mechanisms and signal transduction pathways. Cardiovasc Res 2000;47:23-37.[Abstract/Free Full Text]
  • Swynghedauw B. Molecular mechanisms of myocardial remodeling. Physiol Rev 1999;79:215-62.[Abstract/Free Full Text]
  • Neyses L., Pelzer T. The biological cascade leading to cardiac hypertrophy. Eur Heart J 1995;16:8-11.[Abstract]
  • Chien KR, Knowlton KU, Zhu H., Chien S. Regulation of cardiac gene expression during myocardial growth and hypertrophy: molecular studies of an adaptive physiologic response. FASEB J 1991;5:3037-46.[Abstract]
  • Izumo S., Nadal-Ginard B., Mahdavi V. Protooncogene induction and reprogramming of cardiac gene expression produced by pressure overload. Proc NatlAcad Sci USA 1988;85:339-43.[Abstract/Free Full Text]
  • Baker KM, Booz GW, Dostal DE Cardiac actions of angiotensin II: Role of an intracardiac renin-angiotensin system. Annu Rev Physiol 1992;54:227-41.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Goodfriend TL, Elliott ME, Catt KJ Angiotensin receptors and their antagonists. N Engl JMed 1996;334:1649-54.[Free Full Text]
  • Schlaich MP, Schmieder RE Left ventricular hypertrophy and its regression: pathophysiology and therapeutic approach: focus on treatment by antihypertensive agents. Am J Hypertens 1998;11:1394-404.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Roman MJ,Alderman MH, Pickering TG et al. Differential effects of angiotensin converting enzyme inhibition and diuretic therapy on reductions in ambulatory blood pressure, left ventricular mass, and vascular hypertrophy. Am J Hypertens 1998;11:387-96.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Mullins JJ, Peters J., Ganten D. Fulminant hypertension in transgenic rats harbouring the mouse Ren-2 gene. Nature 1990;344:541-4.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Lee MA, Bohm M., Kim S. et al. Differential gene expression of renin and angiotensinogen in the TGR(mREN-2)27 transgenic rat.Hypertension 1995;25:570-80.[Abstract/Free Full Text]
  • Campbell DJ, Rong P., Kladis A., Rees B., Ganten D., Skinner SL Angiotensin and bradykinin peptides in the TGR(mRen-2)27 rat. Hypertension 1995;25:1014-20.[Abstract/Free Full Text]
  • Ohta K., Kim S., Wanibuchi H., Ganten D., Iwao H. Contribution of local renin-angiotensin system to cardiac hypertrophy, phenotypic modulation, and remodeling in TGR (mRen2)27 transgenic rats. Circulation 1996;94:785-91.[Abstract/Free Full Text]
  • Sandbrink R., Masters CL, Beyreuther K. Similar alternative splicing of a non-homologous domain in beta A4-amyloid protein precursor-like proteins. J Biol Chem 1994;269:14227-34.[Abstract/Free Full Text]
  • BayerTA, Cappai R., Masters CL, Beyreuther K., Multhaup G. It all sticks together: the APP-related family of proteins and Alzheimer's disease. Mol Psychiatry 1999;4:524-8.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Tusher VG, Tibshirani R., Chu G. Significance analysis of microarrays applied to the ionizing radiation response. Proc Natl Acad Sci USA 2001;98:5116-21.[Abstract/Free Full Text]
  • Schonherr E., Witsch-Prehm P., Harrach B., Robenek H., Rauterberg J., Kresse H. Interaction of biglycan with type I collagen. J Biol Chem 1995;270:2776-83.[Abstract/Free Full Text]
  • HildebrandA, Romaris M., Rasmussen LM et al. Interaction of the small interstitial proteoglycans biglycan, decorin and fibromodulin with transforming growth factor beta. Biochem J 1994;302:527-34.[Web of Science][Medline] [Order article via Infotrieve]
  • Shihab FS, Andoh TF, Tanner AM, Bennett WM Sodium depletion enhances fibrosis and the expression of TGF-beta1 and matrix proteins in experimental chronic cyclosporine nephropathy. Am J Kidney Dis 1997;30:71-81.[Web of Science][Medline] [Order article via Infotrieve]
  • AyadaY., Kusachi S., Murakami T. et al. Increased expression of biglycan mRNA in pressure-overloaded rat heart. Clin Exp Hypertens 2001;23:633-43.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Ahmed MS, Oie E., Vinge LE et al. Induction of myocardial biglycan in heart failure in rats--an extracellular matrix component targeted by AT(1) receptor antagonism. Cardiovasc Res 2003;60:557-68.[Abstract/Free Full Text]
  • Hayashi T., Hirshman MF, Kurth EJ, Winder WW, Goodyear LJ Evidence for 5' AMP-activated protein kinase mediation of the effect of muscle contraction on glucose transport. Diabetes 1998;47:1369-73.[Abstract]
  • Kudo N., Gillespie JG, Kung L. et al. Characterization of 5'AMP-activated protein kinase activity in the heart and its role in inhibiting acetyl-CoA carboxylase during reperfusion following ischemia. Biochim Biophys Acta 1996;1301:67-75.[Medline] [Order article via Infotrieve]
  • Tian R., Musi N., D'Agostino J., Hirshman MF, Goodyear LJ Increased adenosine monophosphate-activated protein kinase activity in rat hearts with pressure-overload hypertrophy. Circulation 2001;104:1664-9.[Abstract/Free Full Text]
  • Sambandam N., Lopaschuk GD AMP-activated protein kinase (AMPK) control of fatty acid and glucose metabolism in the ischemic heart. Prog Lipid Res 2003;42:238-56.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Coven DL, Hu X., Cong L. et al. Physiological role of AMP-activated protein kinase in the heart: graded activation during exercise. Am J Physiol Endocrinol Metab 2003;285:629-36.
  • Daniel T., Carling D. Functionalanalysis of mutations in the gamma 2 subunit of AMP-activated protein kinase associated with cardiac hypertrophy and Wolff-Parkinson-White syndrome.JBiol Chem 2002;277:51017-24.[Abstract/Free Full Text]
  • Ono K., Iwanaga Y., Mannami T. et al. Epidemiological evidence of an association between SLC6A2 gene polymorphism and hypertension. Hypertens Res 2003;26:685-9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • MohriT., Emoto N., Nonaka H. et al.Alterations of circadian expressions of clock genes in Dahl salt-sensitive rats fed a high-salt diet. Hypertension 2003;42:189-94.[Abstract/Free Full Text]
  • Young ME, Razeghi P., Taegtmeyer H. Clock genes in the heart: characterization and attenuation with hypertrophy. Circ Res 2001;88:1142-50.[Abstract/Free Full Text]
  • Pan L., Glenn ST, Jones CA, Gronostajski RM, Gross KW Regulation of renin enhancer activity by nuclear factor I and Sp1/Sp3. Biochim Biophys Acta 2003;1625:280-90.[Medline] [Order article via Infotrieve]
  • Rossjohn J., Cappai R., Feil SC et al. Crystal structure of the N-terminal, growth factor-like domain of Alzheimer amyloid precursor protein. Nat Struct Biol 1999;6:327-31.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Von Koch CS, Zheng H., Chen H. et al. Generation of APLP2 KO mice and early postnatal lethality in APLP2/APP double KO mice. Neurobiol Aging 1997;18:661-9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Heber S., Herms J., Gajic V. et al. Mice with combined gene knock-outs reveal essential and partially redundant functions of amyloid precursor protein family members. J Neurosci 2000;20:7951-63.[Abstract/Free Full Text]
  • Dempsey AA, Pabalan N., Tang HC, Liew CC Organization of human cardiovascular-expressed genes on chromosomes 21 and 22. J Mol Cell Cardiol 2001;33:587-91.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Lis CG, Gaviria M. Vascular dementia, hypertension, and the brain. Neurol Res 1997;19:471-80.[Web of Science][Medline] [Order article via Infotrieve]
  • Skoog I. The relationship between blood pressure and dementia: a review. Biomed Pharmacother 1997;51:367-75.[CrossRef][Medline] [Order article via Infotrieve]
  • Farrer LA, Sherbatich T., Keryanov SA et al. Association between angiotensin-converting enzyme and Alzheimer disease. Arch Neurol 2000;57:210-14.[Abstract/Free Full Text]
  • Crawford F., Abdullah L., Schinka J. et al. Gender-specific association of the angiotensin converting enzyme gene with Alzheimer's disease. Neurosci Lett 2000;280:215-19.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Monastero R., Caldarella R., Mannino M. et al. Lack of association between angiotensin converting enzyme polymorphism and sporadic Alzheimer's disease. Neurosci Lett 2002; P335:147-9.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Pachori AS, Numan MT, Ferrario CM, Diz DM, Raizada MK, Katovich MJ Blood pressure-independent attenuation of cardiac hypertrophy by AT(1)R-AS gene therapy. Hypertension 2002;39:969-75.[Abstract/Free Full Text]
  • Sano H., Okamoto H., Kitabatake A., Iizuka K., Murakami T., Kawaguchi H. Increased mRNA expression of cardiac renin-angiotensin system and collagen synthesis in spontaneously hypertensive rats.Mol Cell Biochem 1998;178:51-8.[CrossRef][Web of Science][Medline] [Order article via Infotrieve]
  • Jurkovicova D., Dobesova Z., Kunes J., Krizanova O. Different expression of renin-angiotensin system components in hearts of normotensive and hypertensive rats. Physiol Res 2001;50: 35-42.[Web of Science][Medline] [Order article via Infotrieve]
  • Sassard J., Lo M., Liu KL Lyon genetically hypertensive rats: an animal model of `low renin hypertension`. Acta Pharmacol Sin 2003;24:1-6.[Web of Science][Medline] [Order article via Infotrieve]

Journal of Renin-Angiotensin-Aldosterone System, Vol. 5, No. 2, 72-78 (2004)
DOI: 10.3317/jraas.2004.013
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