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Renin-angiotensin system expression in the K562 human erythroleukaemic cell line
Ebru Koca
Hacettepe University Medical School, Departments of Internal Medicine and Haematology, Ankara, Turkey, ebrukoca{at}gmail.com
Ibrahim C Haznedaroglu
Hacettepe University Medical School, Departments of Internal Medicine and Haematology, Ankara, Turkey
Kadir Acar
Gazi University Medical School, Department of Haematology, Ankara, Turkey
Yavuz Beyazit
Hacettepe University Medical School, Departments of Internal Medicine and Haematology, Ankara, Turkey
Salih Aksu
Hacettepe University Medical School, Departments of Internal Medicine and Haematology, Ankara, Turkey
Muge Misirlioglu
Metis Biotechnology, Inc., Ankara, Turkey
Serdar Tuncer
Metis Biotechnology, Inc., Ankara, Turkey
Nilgun Sayinalp
Hacettepe University Medical School, Departments of Internal Medicine and Haematology, Ankara, Turkey
Osman I Ozcebe
Hacettepe University Medical School, Departments of Internal Medicine and Haematology, Ankara, Turkey
Aysegul Uner
Hacettepe University Medical School, Department of Pathology, Ankara, Turkey
Local renin-angiotensin system (RAS) may affect leukaemic cell production within the bone marrow microenvironment.Angiotensin-converting enzyme (ACE), renin, and angiotensin could influence leukaemogenesis. In this study, mRNA expressions of the major RAS components (ACE, renin, and angiotensinogen) in K562 human erythroleukaemia cell line have been searched by Real Time quantitative polymerase chain reaction. K562 blasts are multipotential, haematopoietic malignant cells that spontaneously differentiate into recognisable progenitors of the erythrocyte, granulocyte and monocytic series.We observed significant expressions of ACE, renin, and angiotensinogen in K562 leukaemic blast cells.Therefore, K562 human erythroleukaemia cell line may serve as an in vitro model to elucidate the role of RAS in leukaemia and to test the effects of RAS-affecting drugs on leukaemic cellular proliferation.
Key Words: renin-angiotensin system • ACE • renin • angiotensinogen • cell line • K562 human erythroleukaemia
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Journal of Renin-Angiotensin-Aldosterone System, Vol. 8, No. 3,
145-147 (2007)
DOI: 10.3317/jraas.2007.019
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