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Angiotensinogen gene variants in a Pakistani hypertensive population of Punjab

Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus, University of Punjab, Lahore 54590, Pakistan

Shahida Hasnain

Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus, University of Punjab, Lahore 54590, Pakistan, shahida@ mmg.pu.edu.pk

Background. The renin-angiotensin-aldosterone system (RAAS) plays a key role in blood pressure (BP) regulation. Among the components of the RAAS, the gene for the angiotensinogen (AGT) has been extensively studied. Several studies in different populations link Threonine instead of methionine at position 235 (M235T) and Methinine instead of threonine at position 174 (T174M) polymorphisms with essential hypertension. We were unable to study these polymorphisms in the Punjab population of Pakistan through routine Restriction Fragment Length Polymorphism (RFLP) method. Considering the importance of this region we decided to further investigate the 300 bp region harbouring these two single nucleotide polymorphisms.

Methods. Samples were derived from a larger study group. Polymerase chain reaction amplified fragments were subjected to either RFLP or Single Strand Conformation. Polymorphism. Single stranded DNA showing mobility shift on denaturing gel were sequenced.

Results. Sequencing confirmed the presence of M235T and T174M polymorphisms in the local population. In addition to these polymorphisms one additional base was found at an identical position in two of the samples. We found a substitution of G with C just adjacent to T174M polymorphism in all seven of our samples studied.

Conclusions. We report two additional bases and one substitution in the angiotensinogen gene of Punjab population. We also suggest that SsmI can be used for the investigation of T174M polymorphism.

Key Words: angiotensinogen • hypertension • Pakistan • polymorphism • renin-angiotensin-aldosterone system • renin-angiotensin-system • single nucleotide

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Journal of Renin-Angiotensin-Aldosterone System, Vol. 9, No. 1, 27-31 (2008)
DOI: 10.3317/jraas.2008.006


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Alvi, F. M Articles by Hasnain, S. Search for Related Content PubMed PubMed Citation Articles by Alvi, F. M Articles by Hasnain, S. Social Bookmarking                         What's this?