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Up- and down-regulation of angiotensin II AT₁-A and AT₁-B receptors in afferent and efferent rat kidney arterioles

Department of Pathology, University of Szeged, Hungary, razgazst{at}patho.szote. u-szeged.hu

Jens Randel Nyengaard

Stereology and Electron Microscopy Research Laboratory and MIND Centre, University of Aarhus, Denmark

Introduction. The contractile effect of angiotensin II via AT₁ receptors on the kidney arterioles is a crucial element in the kidney microcirculation.Angiotensin II also plays a role as an inhibitor via the AT₁ receptors in the renin granulation of the arterioles.We have previously demonstrated that the AT₁ receptors are downregulated in the renin-positive smooth muscle cells (SMCs) in contrast to renin-negative SMCs. In this study, we estimated the numbers of the AT₁ receptor sub-types separately in the afferent and efferent arterioles and the renin-positive and renin-negative SMCs.

Methods. The immunohistochemical signals of theAT₁-A and AT₁-B receptors were counted by stereological methods.¹

Results. The number of AT₁-B receptors in the efferent arterioles (expressed in signals/µm ³; mean (CV): 0.32 (0.33)) was significantly higher (78%; p<0.05) as compared with the number in the afferent arterioles (0.18 (0.11)). No differences were found in the AT₁-A receptors. In a number ofAT ₁-A receptors, significant differences (p<0.01) were detected between the afferent arteriolar renin-positive SMCs (0.13 (0.36)) and the number in renin-negative SMCs (0.25 (0.34)).The AT₁-B receptors did not display any differences.

Conclusions. These results indicate that the AT₁ receptor sub-types are regulated independently in the SMCs of the normal kidney arterioles.

Key Words: aminopeptidase • blood pressure • oral contraceptive pill

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Journal of Renin-Angiotensin-Aldosterone System, Vol. 9, No. 4, 196-201 (2008)
DOI: 10.1177/1470320308098592


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This Article Abstract Free Full Text (Free PDF) Free Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Saved Citations Download to citation manager Request Permissions Request Reprints Add to My Marked Citations Citing Articles Citing Articles via Google Scholar Citing Articles via Scopus Google Scholar Articles by Razga, Z. Articles by Nyengaard, J. R. Search for Related Content PubMed PubMed Citation Articles by Razga, Z. Articles by Nyengaard, J. R. Social Bookmarking                         What's this?